Journal
ONCOLOGY REPORTS
Volume 39, Issue 6, Pages 2845-2854Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2018.6377
Keywords
spermidine; cervical cancer; proliferation; apoptosis; autophagy
Categories
Funding
- National Natural Science Foundation of China [21503190]
- Education Foundation of Zhejiang province [Y201636954]
- Zhejiang Medical College Youth Dr. start-up funding [2015B07]
- Foundation of Zhejiang province Chinese Medicine, Science and Technology [2017ZB030]
- Medical and Health Science and Technology Project of Zhejiang Province [2018277310]
Ask authors/readers for more resources
Cervical cancer is the most common malignancy of the female reproductive tract, and the poor response to prophylactic vaccines and the toxicity of high-dose chemotherapeutic drugs have limited their clinical application. Spermidine, a natural polyamine detected in all eukaryotic organisms, exhibits functions that promote longevity in multiple model systems and may constitute a promising agent for cancer treatment. However, the potential effectiveness of spermidine in cervical cancer has not yet been fully elucidated, and the underlying molecular mechanisms remain unclear. In the present study, we aimed to assess the effects of spermidine on proliferation and apoptosis of HeLa cells (a cervical cancer cell line). Firstly, CCK-8 and flow cytometric assays revealed that spermidine reduced the proliferation of HeLa cells in a dose-dependent fashion by arresting the cell cycle at the S phase. Secondly, flow cytometry incorporating Annexin V-FITC/PI-staining revealed that spermidine promoted the apoptosis of HeLa cells, and western blot analysis revealed that spermidine activated autophagy. Finally, spermidine-activated autophagy mediated the inhibition of cell proliferation by spermidine and spermidine-induced apoptosis in HeLa cells. Collectively, these results revealed a novel function for spermidine in inhibiting cellular proliferation and inducing apoptosis of HeLa cells by activating autophagy, which may have important implications for the use of spermidine in cervical cancer therapy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available