Journal
NUCLEIC ACIDS RESEARCH
Volume 46, Issue 10, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gky164
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Funding
- Netherlands Organization for Scientific Research ZonMW-TOP [91210033, 91211061]
- European Research Council Advanced Grant [694466]
- Danish Council for independent Research, Medical Sciences [DFF-4183-00599]
- Danish Cancer Society [R91-A7351-14-S9]
- European Research Council [AdG 694466]
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Template-directed CRISPR/Cas9 editing is a powerful tool for introducing subtle mutations in genomes. However, the success rate of incorporation of the desired mutations at the target site is difficult to predict and therefore must be empirically determined. Here, we adapted the widely used TIDE method for quantification of templated editing events, including point mutations. The resulting TIDER method is a rapid, cheap and accessible tool for testing and optimization of template-directed genome editing strategies.
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