Journal
NUCLEIC ACIDS RESEARCH
Volume 46, Issue 17, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gky513
Keywords
-
Categories
Funding
- National Aeronautics and Space Administration [NNX14AK15G]
- Simons Foundation [287624]
Ask authors/readers for more resources
A polymerase ribozyme can be used to label the 3'-end of RNA or DNA molecules by incorporating a variety of functionalized nucleotide analogs. Guided by a complementary template, the ribozyme adds a single nucleotide that may contain a fluorophore, biotin, azide or alkyne moiety, thus enabling the detection and/or capture of selectively labeled materials. Employing a variety of commercially available nucleotide analogs, efficient labeling was demonstrated for model RNAs and DNAs, human microRNAs and natural tRNA.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available