Journal
NEW PHYTOLOGIST
Volume 220, Issue 3, Pages 865-877Publisher
WILEY
DOI: 10.1111/nph.15181
Keywords
anther; cis activity; male sterility; miR2118; phased small interfering RNA (phasiRNA)
Categories
Funding
- NSF Plant Genome Research Program award [1339229]
- National Natural Science Foundation of China (NSFC) [91540101]
- Thousand Talents Program for Young Scholars
- Program for Guangdong Introducing Innovative and Entrepreneurial Team [2016ZT06S172]
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1649424] Funding Source: National Science Foundation
Ask authors/readers for more resources
Post-transcriptional gene silencing in plants results from independent activities of diverse small RNA types. In anthers of grasses, hundreds of loci yield noncoding RNAs that are processed into 21- and 24-nucleotide (nt) phased small interfering RNAs (phasiRNAs); these are triggered by miR2118 and miR2275. We characterized these 'reproductive phasiRNAs' from rice (Oryza sativa) panicles and anthers across seven developmental stages. Our computational analysis identified characteristics of the 21-nt reproductive phasiRNAs that impact their biogenesis, stability, and potential functions. We demonstrate that 21-nt reproductive phasiRNAs can function in cis to target their own precursors. We observed evidence of this cis regulatory activity in both rice and maize (Zea mays). We validated this activity with evidence of cleavage and a resulting shift in the pattern of phasiRNA production. We characterize biases in phasiRNA biogenesis, demonstrating that the Pol II-derived 'top' strand phasiRNAs are consistently higher in abundance than the bottom strand. The first phasiRNA from each precursor overlaps the miR2118 target site, and this impacts phasiRNA accumulation or stability, evident in the weak accumulation of this phasiRNA position. Additional influences on this first phasiRNA duplex include the sequence composition and length, and we show that these factors impact Argonaute loading.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available