Journal
NEW PHYTOLOGIST
Volume 219, Issue 4, Pages 1480-1491Publisher
WILEY
DOI: 10.1111/nph.15231
Keywords
AGAMOUS (AG); CURLY LEAF (CLF); H3K27me3; intronic noncoding RNA (incRNA); second intron
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Funding
- Returning Singaporean Scientists Scheme (RSSS) of the National Research Foundation, Singapore [NRF-RSSS-002]
- Ministry of Science and Technology, Taiwan [102-2917-I-564-017]
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Dispersed H3K27 trimethylation (H3K27me3) of the AGAMOUS (AG) genomic locus is mediated by CURLY LEAF (CLF), a component of the Polycomb Repressive Complex (PRC) 2. Previous reports have shown that the AG second intron, which confers AG tissue-specific expression, harbors sequences targeted by several positive and negative regulators. Using RACE reverse transcription polymerase chain reaction, we found that the AG intron 2 encodes several noncoding RNAs. RNAi experiment showed that incRNA4 is needed for CLF repressive activity. AG-incRNA4RNAi lines showed increased leaf AGmRNA levels associated with a decrease of H3K27me3 levels; these plants displayed AG overexpression phenotypes. Genetic and biochemical analyses demonstrated that the AG-incRNA4 can associate with CLF to repress AG expression in leaf tissues through H3K27me3-mediated repression and to autoregulate its own expression level. The mechanism of AG-incRNA4-mediated repression may be relevant to investigations on tissue-specific expression of Arabidopsis MADS-box genes.
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