Journal
NEW PHYTOLOGIST
Volume 218, Issue 3, Pages 1278-1287Publisher
WILEY
DOI: 10.1111/nph.15097
Keywords
acquired heat tolerance; basal heat tolerance; Chl fluorescence; heat acclimation; heat stress; ion leakage; membrane thermostability
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Funding
- Grant Agency of Czech Republic [13-28093S]
- Technological Agency of Czech Republic [TA01010254]
- Ministry of Education, Youth and Sports of Czech Republic from National Program of Sustainability I [LO1204, LO1415]
- Ministry of Education, Youth and Sports of Czech Republic [CZ.02.1.01/0.0/0.0/16_013/0001609]
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Heat tolerance of plants related to cell membrane thermostability is commonly estimated via the measurement of ion leakage from plant segments after defined heat treatment. To compare heat tolerance of various plants, it is crucial to select suitable heating conditions. This selection is time-consuming and optimizing the conditions for all investigated plants may even be impossible. Another problem of the method is its tendency to overestimate basal heat tolerance. Here we present an improved ion leakage method, which does not suffer from these drawbacks. It is based on gradual heating of plant segments in a water bath or algal suspensions from room temperature up to 70-75 degrees C. The electrical conductivity of the bath/suspension, which is measured continuously during heating, abruptly increases at a certain temperature T-COND (within 55-70 degrees C). The T-COND value can be taken as a measure of cell membrane thermostability, representing the heat tolerance of plants/organisms. Higher T-COND corresponds to higher heat tolerance (basal or acquired) connected to higher thermostability of the cell membrane, as evidenced by the common ion leakage method. The new method also enables determination of the thermostability of photochemical reactions in photosynthetic samples via the simultaneous measurement of Chl fluorescence.
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