Journal
GENOME BIOLOGY
Volume 14, Issue 4, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/gb-2013-14-4-r31
Keywords
Single cell; RNA-seq; Transcriptome; Sequencing; Bioinformatics; Cellular heterogeneity; Cell biology
Funding
- Program for Innovative Cell Biology by Innovative Technology from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan
- Leading Project for the Realization of Regenerative Medicine
- RIKEN CDB
- Special Postdoctoral Researchers Program from RIKEN
- Grants-in-Aid for Scientific Research [23680038] Funding Source: KAKEN
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Development of a highly reproducible and sensitive single-cell RNA sequencing (RNA-seq) method would facilitate the understanding of the biological roles and underlying mechanisms of non-genetic cellular heterogeneity. In this study, we report a novel single-cell RNA-seq method called Quartz-Seq that has a simpler protocol and higher reproducibility and sensitivity than existing methods. We show that single-cell Quartz-Seq can quantitatively detect various kinds of non-genetic cellular heterogeneity, and can detect different cell types and different cell-cycle phases of a single cell type. Moreover, this method can comprehensively reveal gene-expression heterogeneity between single cells of the same cell type in the same cell-cycle phase.
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