Journal
BIOSENSORS-BASEL
Volume 3, Issue 3, Pages 341-359Publisher
MDPI
DOI: 10.3390/bios3030341
Keywords
perfluorocarbon; nanoemulsion; F-19 MRI; imaging; stability; cell labeling
Funding
- National Institutes of Health [P41 EB001977, R41-EB009618]
- Center for Molecular Analysis (Carnegie Mellon University)
- NSF [CHE-0130903, CHE-1039870]
- Commonwealth Universal Research Enhancement (C.U.R.E.) program from the Pennsylvania Department of Health
Ask authors/readers for more resources
This report describes novel perfluorocarbon (PFC) nanoemulsions designed to improve ex vivo cell labeling for F-19 magnetic resonance imaging (MRI). 19F MRI is a powerful non-invasive technique for monitoring cells of the immune system in vivo, where cells are labeled ex vivo with PFC nanoemulsions in cell culture. The quality of 19F MRI is directly affected by the quality of ex vivo PFC cell labeling. When co-cultured with cells for longer periods of time, nanoemulsions tend to settle due to high specific weight of PFC oils (1.5-2.0 g/mL). This in turn can decrease efficacy of excess nanoemulsion removal and reliability of the cell labeling in vitro. To solve this problem, novel PFC nanoemulsions are reported which demonstrate lack of sedimentation and high stability under cell labeling conditions. They are monodisperse, have small droplet size (similar to 130 nm) and low polydispersity (<0.15), show a single peak in the 19F nuclear magnetic resonance spectrum at -71.4 ppm and possess high fluorine content. The droplet size and polydispersity remained unchanged after 160 days of follow up at three temperatures (4, 25 and 37 degrees C). Further, stressors such as elevated temperature in the presence of cells, and centrifugation, did not affect the nanoemulsion droplet size and polydispersity. Detailed synthetic methodology and in vitro testing for these new PFC nanoemulsions is presented.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available