Journal
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume 36, Issue 19, Pages 2749-2757Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826076.2012.725694
Keywords
chromatographic fingerprint; Glycyrrhizae radix; ultra-fast liquid chromatography
Funding
- National Scientific & Technological Special Project-Major Creation of New Drugs [2010ZX09401-306-1-4, 2010ZX09401-306-2-19]
Ask authors/readers for more resources
In the present study, a method based on ultra-fast liquid chromatography (UFLC) was successfully developed for chromatographic fingerprint (CFP) analysis of Glycyrrhizae radix. The CFP analysis was performed on an Agilent ZorBax XDB-C-18 column (2.1x100mm, 1.8 mu m) using a gradient elution of methanol and water (containing 1% acetic acid) within a runtime of 30min. The method was validated by eleven common peaks that were shown in all samples. The results revealed that the method was precise, stable, and reproducible. Using the established method, three Glycyrrhizae radix samples from different sources were evaluated for their similarity. Compared to the conventional high performance liquid chromatography, UFLC provided many advantages, such as time saving, less solvent consumption and better separation efficiency. It was a reliable and powerful method for quality control of traditional Chinese medicines with multi-components.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available