Journal
NATURE METHODS
Volume 15, Issue 7, Pages 527-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41592-018-0037-8
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Funding
- German Research Foundation (DFG/Gottfried Wilhelm Leibniz Prize)
- European Union [686547]
- Max-Planck Society for the Advancement of Sciences
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We developed EASI-tag (easily abstractable sulfoxide-based isobaric-tag), a new type of amine-derivatizing and sulfoxide- containing isobaric labeling reagents for highly accurate quantitative proteomics analysis using mass spectrometry. We observed that EASI-tag labels dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of C-12 precursors and quantification at the MS2 level allowed accurate determination of quantitative differences between up to six multiplexed samples.
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