Journal
NATURE METHODS
Volume 15, Issue 6, Pages 425-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-018-0004-4
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Funding
- Intramural Research Programs of the National Institute of Biomedical Imaging and Bioengineering
- National Heart, Lung, and Blood Institute
- NSF [1607645]
- NIH Director's Challenge Innovation Award Program
- Division Of Physics
- Direct For Mathematical & Physical Scien [1607645] Funding Source: National Science Foundation
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We combined instant structured illumination microscopy (iSIM) with total internal reflection fluorescence microscopy (TIRFM) in an approach referred to as instant TIRF-SIM, thereby improving the lateral spatial resolution of TIRFM to 115 +/- 13 nm without compromising speed, and enabling imaging frame rates up to 100 Hz over hundreds of time points. We applied instant TIRF-SIM to multiple live samples and achieved rapid, high-contrast super-resolution imaging close to the coverslip surface.
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