Journal
NATURE METHODS
Volume 15, Issue 6, Pages 433-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-018-0006-2
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Funding
- US National Institutes of Health (4D Nucleome grant) [U54 DK107980]
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Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 biotinylation at genomic elements by restricted spatial tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, thereby facilitating annotation of these factors and their roles.
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