Journal
NATURE METHODS
Volume 15, Issue 8, Pages 617-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-018-0044-9
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Funding
- ERC CoG Peroxisystem [646604]
- DFG [SFB 1190]
- Mitzutani foundation
- VolksWagen foundation [93092]
- DIP grant [P17516]
- Foundation Grant from the Canadian Institutes of Health Research [FDN-143289]
- Canadian Institutes of Health Research [MOP-GMX-152556]
- Israel Science Foundation [1775/12, 2179/14]
- Azrieli student-award grant
- European Research Council (ERC) [646604] Funding Source: European Research Council (ERC)
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Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of similar to 5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.
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