Journal
NATURE METHODS
Volume 15, Issue 5, Pages 355-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.4639
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Funding
- Alexander-von-Humboldt Stiftung (Humboldt Professorship)
- Sachsisches Ministerium fur Wissenschaft und Kunst [TG70]
- ERC (starting grant LightTouch) [282060]
- DFG Center for Regenerative Medicine of the Technische Universitat Dresden
- DFG [KFO249, GU 612/2-2]
- DKMS ('Mechthild Harf Research Grant') [DKMS-SLS-MHG-2016-02]
- Cancer Research UK [C1529/A17343]
- Sachsisches Ministerium fur Wissenschaft und Kunst (European Fund for Regional Development-EFRE)
- Light Microscopy Facility
- Microstructure Facility
- Biotechnology and Biological Sciences Research Council [BB/K009001/1] Funding Source: researchfish
- Cancer Research UK [23335, 17343, 9786] Funding Source: researchfish
- Medical Research Council [MC_U12266B, MC_CF12266, 1214605] Funding Source: researchfish
- BBSRC [BB/K009001/1] Funding Source: UKRI
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The throughput of cell mechanical characterization has recently approached that of conventional flow cytometers. However, this very sensitive, label-free approach still lacks the specificity of molecular markers. Here we developed an approach that combines real-time 1D-imaging fluorescence and deformability cytometry in one instrument (RT-FDC), thus opening many new research avenues. We demonstrated its utility by using subcellular fluorescence localization to identify mitotic cells and test for mechanical changes in those cells in an RNA interference screen.
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