Journal
NATURE CHEMICAL BIOLOGY
Volume 14, Issue 3, Pages 299-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NCHEMBIO.2555
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Funding
- MEXT/JSPS KAKENHI [JP26291057, JP16H01237, JP17H06476, JP16H01462, JP17H03695, JP17KT0017, JP26440140, JP15H05956, JP17H06350, JP16H01472, S1511023]
- Japan Science and Technology Agency (PRESTO) [JPMJPR15Q9]
- Japan Science and Technology Agency (ERATO) [JPMJER1302]
- Howard Hughes Medical Institute (HHMI)
- Gordon and Betty Moore Foundation [GBMF3035]
- Grants-in-Aid for Scientific Research [17H03695, 15H05955] Funding Source: KAKEN
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The phytohormone auxin indole-3-acetic acid (IAA) regulates nearly all aspects of plant growth and development. Despite substantial progress in our understanding of auxin biology, delineating specific auxin response remains a major challenge. Auxin regulates transcriptional response via its receptors, TIR1 and AFB F-box proteins. Here we report an engineered, orthogonal auxin-TIR1 receptor pair, developed through a bump-and-hole strategy, that triggers auxin signaling without interfering with endogenous auxin or TIR1/AFBs. A synthetic, convex IAA (cvxIAA) hijacked the downstream auxin signaling in vivo both at the transcriptomic level and in specific developmental contexts, only in the presence of a complementary, concave TIR1 (ccvTIR1) receptor. Harnessing the cvxIAA-ccvTIR1 system, we provide conclusive evidence for the role of the TIR1-mediated pathway in auxin-induced seedling acid growth. The cvxIAA-ccvTIR1 system serves as a powerful tool for solving outstanding questions in auxin biology and for precise manipulation of auxin-mediated processes as a controllable switch.
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