Journal
NATURE BIOTECHNOLOGY
Volume 36, Issue 4, Pages 324-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.4102
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Funding
- MOST [2014CB910600]
- NSFC [31600619, 31600654, 31471241, 31730111, 91540115]
- Shanghai Municipal Science and Technology Commission [16PJ1407000, 16PJ1407500]
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The targeting range of CRISPR-Cas9 base editors (BEs) is limited by their G/C-rich protospacer-adjacent motif (PAM) sequences. To overcome this limitation, we developed a CRISPR-Cpf1-based BE by fusing the rat cytosine deaminase APOBEC1 to a catalytically inactive version of Lachnospiraceae bacterium Cpf1. The base editor recognizes a T-rich PAM sequence and catalyzes C-to-T conversion in human cells, while inducing low levels of indels, non-C-to-T substitutions and off-target editing.
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