Journal
NATURE BIOTECHNOLOGY
Volume 36, Issue 6, Pages 505-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.4132
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Funding
- Carl R. Woese Institute for Genomic Biology at the University of Illinois at Urbana-Champaign
- US Department of Energy [DE-SC0018260]
- U.S. Department of Energy (DOE) [DE-SC0018260] Funding Source: U.S. Department of Energy (DOE)
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We developed a CRISPR-Cas9- and homology-directed-repair-assisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited with an average frequency of 82%. We validate the single-nucleotide resolution genome-editing capability of this technology by creating a genome-wide gene disruption collection and apply our method to improve tolerance to growth inhibitors.
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