Anti-breast cancer triterpenoid saponins from the thorns of Gleditsia sinensis

One new triterpenoid saponin (1), as well as six known ones (2-7), were isolated from the ethanol extract of the thorns of Gleditsia sinensis. Their structures were elucidated by extensive spectroscopic analysis in conjunction with chemical evidence. Cytotoxic activity of compounds 1-6 was evaluated against human breast cancer MCF 7 cells in vitro by the MTT method. Our results revealed moderate activities for compounds 1-6 with IC50 values of 18.43, 30.47, 18.46, 10.02, 30.76, and 17.32 mu M, respectively. Furthermore, compounds 1, 3, 4, and 6 induced apoptosis in MCF 7 cell, with 1 and 6 causing late apoptosis of MCF 7 cells, while 3 and 4 acting oppositely. [GRAPHICS] .