Journal
NANOSCALE
Volume 10, Issue 3, Pages 881-906Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7nr08360b
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Funding
- British Council/Newton Fund, Brazil [203855854]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil [2012/24574-3, 2015/50257-3, 2014/26897-0, 2014/06863-3]
- Associacao Beneficente Alzira Denise Hertzog Silva (ABADHS), Brazil
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil [441878/2014-8, 202077/2015-2]
- Ricerca Finalizzata from the Ministero della Salute, Italy [172/GR-2011-02350301]
- Science without Borders/CAPES, Brazil [BEX 7057/15-6]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [12/24574-3] Funding Source: FAPESP
- BBSRC [BB/S00324X/1, BB/P006205/1, BB/E002080/1, BB/M006298/1] Funding Source: UKRI
- MRC [MR/N01037X/1] Funding Source: UKRI
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Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nano-sized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.
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