Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 14, Issue 6, Pages 1931-1939Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nano.2018.05.009
Keywords
Cancer stem cell; Array-based sensing; Discrimination; Patient-derived xenografts; Personalized medicine
Funding
- National Institutes of Health [GM077173, CA168464, CA203439, CA207932]
- JSPS Research Fellowships for Young Scientists
- University of Massachusetts President's Science and Technology fund
- NATIONAL CANCER INSTITUTE [R01CA168464, R56CA207932] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM077173] Funding Source: NIH RePORTER
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Cancer stem cells (CSCs) contribute to multidrug resistance, tumor recurrence and metastasis, making them prime therapeutic targets. Their ability to differentiate and lose stem cell properties makes them challenging to study. Currently, there is no simple assay that can quickly capture and trace the dynamic phenotypic changes on the CSC surface. Here, we report rapid discrimination of breast CSCs from non-CSCs using a nanoparticle-fluorescent-protein based sensor. This nanosensor was employed to discriminate CSCs from non-CSCs, as well as CSCs that had differentiated in vitro in two breast cancer models. Importantly, the sensor platform could also discriminate CSCs from the bulk population of cells in patient-derived xenografts of human breast cancer. Taken together, the results obtained demonstrate the feasibility of using the nanosensor to phenotype CSCs and monitor their fate. Furthermore, this approach provides a novel area for therapeutic interventions against these challenging targets. (C) 2018 Elsevier Inc. All rights reserved.
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