Antibiofilm effect of drug-free and cationic poly(D,L-Iactide-co-glycolide) nanoparticles via nano bacteria interactions

Aim: Recently, nano-bio interactions and their biomedical impacts have drawn much attention, but nano bacteria interaction and its function are unknown. Herein, we aim to synthesize drug-free and cationic nanoparticles (CNPs) and investigate CNP-bacteria interaction and its antibiofilm effect. Materials & methods: The bioactivity of CNPs against Streptococcus mutans was examined by colony-forming units counting and scanning electron microscopy. CNP-bacteria interaction force was measured by atomic force microscopy. Results: CNPs (217.7 nm, 14.7 mv) showed a concentration-dependent activity against bacteria. Particularly, CNPs at 200 mu g/ml completely inhibited planktonic bacterial growth and biofilm formation, and disrupted similar to 70% mature biofilm. CNP-bacteria interaction force was up to 184 nN. Conclusion: CNPs have great potentials for convenient local use for prevention and treatment of bacteria-related oral diseases.