Journal
NANOMEDICINE
Volume 13, Issue 7, Pages 749-767Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/nnm-2017-0335
Keywords
cartilage breakdown; cartilage-targeting; cellular uptake; chondrocyte affinity peptide; chondrocytes; intra-articular injection; in vivo imaging; osteoarthritis; polyamidoamine (PAMAM) dendrimer
Funding
- Natural Science Foundation of Jiangsu Province [BK20151224]
- Suzhou Science and Technology Development Project [SYS201713]
- National Natural Science Foundation of China [81302719]
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Aim: To develop a nanocarrier for targeted delivery of agents to the cartilage. Materials & methods: Chondrocyte affinity peptide modified PEGylated polyamidoamine conjugates (CAP-PEG-PAMAM) were prepared and rhodamine B isothiocyanate (RB) fluorophore was linked on them for comparative biological tracing and profiling. Results: CAP4-PP-RB exhibited much more efficient cellular uptake in vitro than that of PEG-PAMAM-RB. Both the conjugates were likely internalized by chondrocytes via clathrin and caveolin co-mediated endocytosis, and delivered to lysosomes. In vivo imaging demonstrated the fluorescein-labeled nanocarrier was capable to persist in the joint cavity of rats for a prolonged time. Furthermore, the CAP4-PEG-PAMAM showed a good biocompatibility and enhanced penetration effects in vivo. Conclusion: CAP-PEG-PAMAM could be an effective nanocarrier for intra-articular delivery of agents to cartilage.
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