Journal
NANOMEDICINE
Volume 13, Issue 6, Pages 605-624Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/nnm-2017-0292
Keywords
apoptosis; brain tumor; colorectal tumor; conjugated polymer nanoparticles; metallated porphyrin; photodynamic therapy; ROS
Funding
- CONICET [PIP 11220150100069, 11220150100295/2015]
- ANPCyT [PICT 1439/13, 914/14, 214/14]
- Instituto Nacional del Cancer [1006/2016]
- SECyT-UNRC [PPI 2016 18/6487]
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Aim: Assess biocompatibility, uptake and photodynamic therapy (PDT) mechanism of metallated porphyrin doped conjugated polymer nanoparticles (CPNs) in human brain and colorectal tumor cells and macrophages. Materials & methods: CPNs were developed employing 9,9-dioctylfluorene-alt-benzothiadiazole, an amphiphilic polymer (PS-PEG-COOH), and platinum octaethylporphyrin. T98G, SW480 and RAW 264.7 cell lines were exposed to CPNs to assess uptake and intracellular localization. Additionally, a PDT protocol using CPNs was employed for the in vitro killing of cancer and macrophage cell lines. Results & conclusion: CPNs were well incorporated into glioblastoma and macrophage cells with localization in lysosomes. SW480 cells were less efficient incorporating CPNs with localization in the plasma membrane. In all cell lines PDT treatment was efficient inducing oxidative stress that triggered apoptosis.
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