Journal
NANO RESEARCH
Volume 11, Issue 6, Pages 3164-3174Publisher
TSINGHUA UNIV PRESS
DOI: 10.1007/s12274-017-1721-1
Keywords
upconversion nanoparticles; polydopamine; Forster resonance energy transfer; blood glucose; bioassay
Categories
Funding
- National Basic Research Program of China (973 Program) [2014CB845605]
- National Natural Science Foundation of China (NSFC) [U1305244, 21325104, 51402294, 21501180]
- CAS/SAFEA International Partnership Program for Creative Research Teams
- Strategic Priority Research Program of the CAS [XDA09030307, XDB20000000]
- Youth Innovation Promotion Association [2016277]
- Chunmiao Project of Haixi Institute of the CAS [CMZX-2016-002]
- Natural Science Foundation of Fujian Province [2015J05116, 2017J05095, 2017J01105, 2017I0018]
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The accurate detection of blood glucose is of critical importance in the diagnosis and management of diabetes and its complications. Herein, we report a novel strategy based on an upconversion nanoparticles-polydopamine (UCNPs-PDA) nanosystem for the accurate detection of glucose in human serum and whole blood through a simple blending of test samples with ligand-free UCNPs, dopamine, and glucose oxidase (GOx). Owing to the high affinity of lanthanide ions exposed on the surface of ligand-free UCNPs, dopamine monomers could spontaneously attach to the UCNPs and further polymerize to form a PDA shell, resulting in a remarkable upconversion luminescence (UCL) quenching (97.4%) of UCNPs under 980-nm excitation. Such UCL quenching can be effectively inhibited by H2O2 produced from the GOx/glucose enzymatic reaction, thus enabling the detection of H2O2 or glucose based on the UCL quenching/inhibition bioassay. Owing to the highly sensitive UCL response and background-free interference of the UCNPs-PDA nanosystem, we achieved a sensitive, selective, and high-throughput bioassay for glucose in human serum and whole blood, thereby revealing the great potential of the UCNPs-PDA nanosystem for the accurate detection of blood glucose or other H2O2-generated biomolecules in clinical bioassays.
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