Journal
NANO LETTERS
Volume 18, Issue 7, Pages 4279-4284Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.8b01267
Keywords
RNA nanostructures; Dicer substrate RNA; gene silencing; enzymatic synthesis; programmable gene regulation
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Funding
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning Pioneer Research Center Program [2014M3C1A3054153]
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future GiRC Program [2012K1A1A2A01056092]
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Basic Science Research Program [2015R1A1A1A05027352]
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future MRC Program [2018R1A5A2025286]
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Korea Bio Grand Challenge Program [2018M3A9H3020844]
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Enzymatic synthesis of RNA nanostructures is achieved by isothermal rolling circle transcription (RCT). Each arm of RNA nanostructures provides a functional role of Dicer substrate RNA inducing sequence specific RNA interference (RNA. Three different RNAi sequences (GFP, RFP, and BFP) are incorporated within the three-arm junction RNA nanostructures (Y-RNA). The template and helper DNA strands are designed for the large-scale in vitro synthesis of RNA strands to prepare self-assembled Y-RNA. Interestingly, Dicer processing of Y-RNA is highly influenced by its physical structure and different gene silencing activity is achieved depending on its arm length and overhang. In addition, enzymatic synthesis allows the preparation of various Y-RNA structures using a single DNA template offering on demand regulation of multiple target genes.
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