Journal
ACTA CHIMICA SLOVACA
Volume 6, Issue 2, Pages 206-210Publisher
DE GRUYTER POLAND SP ZOO
DOI: 10.2478/acs-2013-0033
Keywords
simulated moving bed chromatography; galacto-oligosaccharides; adsorption isotherm
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Funding
- Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic [ITMS 26240220057]
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A mixture of galacto-oligosaccharides and non-prebiotic sugars was separated using a simulated moving bed unit equipped with eight packed-bed columns of a cation exchanger. In order to determine operating conditions using triangle theory, equilibrium adsorption isotherms of galactose, glucose, lactose, tri- and tetragalacto-oligosaccharide were measured. It was found that each column of the SMB unit had a maximum separation efficiency of more than 3000 theoretical plates. The achieved purity of galacto-oligosaccharides in the raffinate stream was 99.9 %, similarly 99.9 % of extract was formed by non-prebiotic sugars lactose, glucose and galactose. The productivities in the raffinate and extract, were 0.6 g/(L h) and 2.4 g/(L h), respectively.
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