Journal
MOLECULES
Volume 23, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/molecules23061287
Keywords
gelatin hydrolysate; human fetal osteoblasts; proliferation; apoptosis; differentiation; Wnt/beta-catenin pathway
Funding
- Program of International Science & Technology Cooperation of Heilongjiang Province [WB13C102]
- National Natural Science Foundation of China [31371738]
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Gelatins from the skin of bovine, porcine, and tilapia were hydrolyzed to three degrees of hydrolysis (DH) by alcalase, neutrase, and papain, respectively. These hydrolysates at 0.02-0.1 g/L promoted the growth of human fetal osteoblasts by 101.4-135.7%, while higher DH or using papain and tilapia gelatins resulted in higher proliferation. The hydrolysates from porcine and tilapia gelatins at 0.05 g/L prevented induced apoptosis (decreasing total apoptotic proportions from 28.4% or 35.2% to 10.3-17.5% or 16.0-23.6%), and had differentiation induction (increasing alkaline phosphatase activity by 126.9-246.7% in early differentiation stage, or enhancing osteocalcin production by 4.1-22.5% in later differentiation stage). These hydrolysates had a similar amino acid profile; however, tilapia gelatin hydrolysates by papain with DH 15.4% mostly displayed higher activity than others. Tilapia gelatin hydrolysate could up-regulate beta-catenin, Wnt 3a, Wnt 10b, cyclin D1, and c-Myc expression at mRNA levels by 1.11-3.60 folds, but down-regulate GSK 3 beta expression by 0.98 fold. Of note, fi -catenin in total cellular and nuclear protein was up-regulated by 1.14-1.16 folds but unchanged in cytoplasmic protein, Wnt 10b, cyclin D1, and c-Myc expression were up-regulated by 1.27-1.95 folds, whilst GSK 3 beta expression was down-regulated by 0.87 fold. Activation of Wnt/beta-catenin pathway is suggested to mediate cell proliferation and differentiation.
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