Journal
MOLECULES
Volume 23, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/molecules23030623
Keywords
Salvia prattii; antibacterial diterpenes; hydrophilic solid-phase extraction; preparative high-performance liquid chromatography
Funding
- High and New Technology Research and Development Planning, Significant Science & Technological Project of Qinghai Province [2014-GX-A3A]
- Natural Science Funds of Qinghai Province [2016-ZJ-933Q]
- Project of Discovery, Evaluation and Transformation of Active Natural Compounds, Strategic Biological Resources Service Network Programme of Chinese Academy of Sciences [ZSTH-027]
- West Light Foundation of the Chinese Academy of Sciences for Doctors
- Youth Innovation Promotion Association of Chinese Academy of Sciences [2017471]
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An efficient preparative procedure for the separation of four antibacterial diterpenes from a Salvia prattii crude diterpenes-rich sample was developed. Firstly, the XION hydrophilic stationary phase was chosen to separate the antibacterial crude diterpenes-rich sample (18.0 g) into three fractions with a recovery of 46.1%. Then, the antibacterial fractions I (200 mg), II (200 mg), and III (150 g) were separated by the Megress C18 preparative column, and compounds tanshinone IIA (80.0 mg), salvinolone (62.0 mg), cryptotanshinone (70.0 mg), and ferruginol (68.0 mg) were produced with purities greater than 98%. The procedure achieved large-scale preparation of the four diterpenes with high purity, and it could act as a reference for the efficient preparation of active diterpenes from other plant extracts.
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