Journal
MOLECULES
Volume 23, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/molecules23030578
Keywords
honey; H-1-NMR fingerprint; profiling; chemometrics; principal component analysis (PCA); partial least squares-discriminant analysis (PLS-DA); grouping
Funding
- Rhodes University
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Honey is the natural sweet substance produced by honeybee from nectar or honeydew, exhibiting several nutritional and health benefits. It contains a complex mixture of compounds in different proportions, with sugars being the main component. The physicochemical characteristics of ten honeys were evaluated; represented by five, three, and two from South Africa, Slovakia, and Zambia, respectively. The range of values for the pH (3.75-4.38), electrical conductivity (99-659 mu S/cm), and moisture content (14.2-17.7%) are within the recommended limits for quality honeys. H-1-NMR (Nuclear Magnetic Resonance) profiling of the honeys in D2O was determined, and the data were analysed by chemometrics. This method is fast, reproducible, and sample pre-treatment is not necessary. The H-1-NMR fingerprints of various chemical shift regions showed similarity or dissimilarity across geographical origins that are useful for identification, detection of adulteration, and quality control. The principal component analysis PCA and partial linear square discriminant analysis PLS-DA of the H-1-NMR profiles successively categorises the honeys into two chemically related groups. The R-2 values are higher than the corresponding Q(2) values for all samples, confirming the reliability of the model. Honeys in the same cluster contain similar metabolites and belong to the same botanic or floral origin.
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