Journal
MOLECULES
Volume 23, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/molecules23010237
Keywords
hnRNPA1; RNA G-quadruplex; telomere RNA; loop of RNA G-quadruplex; base
Funding
- Kohnan Asia Scholarship Foundation
- JSPS KAKENHI [17H03091]
- Takeda Science Foundation
- Grants-in-Aid for Scientific Research [17H03091] Funding Source: KAKEN
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Human telomere RNA performs various cellular functions, such as telomere length regulation, heterochromatin formation, and end protection. We recently demonstrated that the loops in the RNA G-quadruplex are important in the interaction of telomere RNA with heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1). Here, we report on a detailed analysis of hnRNPA1 binding to telomere RNA G-quadruplexes with a group of loop variants using an electrophoretic mobility shift assay (EMSA) and circular dichroism (CD) spectroscopy. We found that the hnRNPA1 binds to RNA G-quadruplexes with the 2'-O-methyl and DNA loops, but fails to bind with the abasic RNA and DNA loops. These results suggested that hnRNPA1 binds to the loop of the RNA G-quadruplex by recognizing the base of the loop's nucleotides. The observation provides the first evidence that the base of the loop's nucleotides is a key factor for hnRNPA1 specifically recognizing the RNA G-quadruplex.
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