Journal
MOLECULAR PAIN
Volume 14, Issue -, Pages -Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/1744806918788681
Keywords
Cancer-induced bone pain; chemokines; MCP-1; CCR2 axis; NF-kappa B signaling pathway; astrocytes; neurons
Categories
Funding
- National Natural Science Foundation of China [81341035, 81171057]
- Natural Science Foundation of Zhejiang Provincial [LY16H090016, LY17H090019]
- Zhejiang provincial and Ministerial Cultivation Plan for Medicine and Health [2015PYA010]
- Zhejiang Provincial Program for the Cultivation of High- level Innovative Health Talents [2012-RC-22]
- Construction Project of Anesthesiology Discipline Special Disease Center in Zhejiang North Region [201524]
- Jiaxing city science and technology project [2017AY33008]
Ask authors/readers for more resources
Background: Chemokine, monocyte chemoattractant protein-1 (MCP-1), is a potential factor to cause cancer-induced bone pain (CIBP). NF-kappa B signaling is very important in mediating the expression of chemokines and may have a role in CIBP. However, the mechanism is still unclear. This study investigates the role of NF-kappa B in CIBP by regulating MCP-1/chemokine CC motif receptor-2 (CCR2) signaling pathway. Methods: A rat CIBP model was established by injecting Walker-256 cells into the tibia medullary cavity. Nine days later, animals were intrathecally administrated with MCP-1 neutralizing antibody, CCR2 antagonist (RS504393), or NF-kappa B inhibitor (BAY11-7081). Mechanical paw withdrawal threshold was used to assess pain behavior and sciatic functional index, and radiographic images were adopted to evaluate the damage of nerve and bone. The spinal cords were harvested for Western blot and quantitative reverse transcription polymerase chain reaction. The distribution of MCP-1, CCR2, and NF-kappa B was detected by double immunofluorescent staining. Results: CIBP caused remarkable bone destruction, injury of sciatic and femoral nerve, and persistent (>15 days) mechanical allodynia in rats. Tumor cell inoculation upregulate MCP-1 and NF-kappa B in activated neurons as well as CCR2 in neurons and microglia of the spinal cord. MCP-1 antibody, RS504393, and BAY11-7081 partially reversed CIBP-induced mechanical allodynia, and CIBP regulated the expression levels of pro-inflammatory cytokines, tumor necrosis factor-alpha and interferon-gamma, and anti-inflammatory cytokine, interleukin 4, and BAY11-7081 lowered CIBP-induced MCP-1 and CCR2 expressions in a dose-dependent manner. Conclusion: In conclusion, NF-kappa B signaling pathway regulates the expressions of MCP-1/CCR2-induced inflammatory factors in the spinal cord of CIBP rats.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available