Journal
MOLECULAR NEUROBIOLOGY
Volume 56, Issue 2, Pages 1461-1474Publisher
SPRINGER
DOI: 10.1007/s12035-018-1158-x
Keywords
Gamma-aminobutyric acid; GABA-A receptor; Peripheral nerve regeneration; Conditional mice
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Funding
- MIUR 'Progetto Eccellenza'
- institutional grant from Universita degli Studi di Milano
- Ministry of Economy and Competitiveness, Spain, MINECO BFU [2016-7798-P]
- Diputacion Foral de Gipuzkoa, Spain [DFG15/15, DFG141/16]
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GABA-B receptors are important for Schwann cell (SC) commitment to a non-myelinating phenotype during development. However, the P0-GABA-B1(fl/fl) conditional knockout mice, lacking the GABA-B1 receptor specifically in SCs, also presented axon modifications, suggesting SC non-autonomous effects through the neuronal compartment. In this in vitro study, we evaluated whether the specific deletion of the GABA-B1 receptor in SCs may induce autonomous or non-autonomous cross-changes in sensory dorsal root ganglia (DRG) neurons. To this end, we performed an in vitro biomolecular and transcriptomic analysis of SC and DRG neuron primary cultures from P0-GABA-B1(fl/fl) mice. We found that cells from conditional P0-GABA-B1(fl/fl) mice exhibited proliferative, migratory and myelinating alterations. Moreover, we found transcriptomic changes in novel molecules that are involved in peripheral neuron-SC interaction.
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