Journal
MOLECULAR MEDICINE REPORTS
Volume 17, Issue 6, Pages 8332-8338Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2018.8880
Keywords
miR-145-5p; hepatocellular carcinoma; Kruppel-like factor 5; proliferation
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Funding
- National Natural Science Foundation of China [81302328]
- Science and Technology Department of Hunan province [2013FJ4059]
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MicroRNAs (miRs) are important in hepatocellular carcinoma (HCC) progression. miR-145-5p acts as a tumor suppressor in certain malignancies, however, its role in HCC remains unclear. The present study aimed to perform a functional analysis of miR-145-5p in HCC in order to elucidate its role in the pathogenesis of HCC. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to analyze tissue and cellular expression of miR-145-5p in HCC. Following miRNA mimics transfection, cell viability, apoptosis and cells migration were determined by Cell Counting kit-8, Annexin V-FITC/propidium iodide staining and Transwell analyses. The target of miR-145-5p was analyzed by luciferase reporter assay and western blot analysis. It was observed that miR-145-5p was significantly decreased in HCC tissues and cell lines. Overexpression of miR-145-5p significantly increased apoptosis, reduced cell proliferation and suppressed HCC cell migration. Kruppel-like factor 5 (KLF5) is regarded as a target of miR-145-5p in HCC cells. In addition, KLF5 overexpression partially attenuated the tumor suppressive effects of miR-145-5p. KLF5 expression was negatively associated with levels of miR-145-5p in HCC tissues. The present study demonstrated that miRNA-145-5p may, by targeting KLF5, partially suppress HCC cell growth and motility. The results of the present study suggested that miRNA-145-5p alteration in HCC may serve a role in the progression of HCC.
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