4.4 Article

PET Imaging Analysis of Vitamin B1 Kinetics with [11C]Thiamine and its Derivative [11C]Thiamine Tetrahydrofurfuryl Disulfide in Rats

Journal

MOLECULAR IMAGING AND BIOLOGY
Volume 20, Issue 6, Pages 1001-1007

Publisher

SPRINGER
DOI: 10.1007/s11307-018-1186-y

Keywords

Vitamin B-1; Thiamine; Fursultiamine; Thiamine tetrahydrofurfuryl disulfide; Positron emission tomography

Funding

  1. Takeda Pharmaceutical Company Limited (Tokyo, Japan)

Ask authors/readers for more resources

PurposeThiamine is an essential component of glucose metabolism and energy production. The disulfide derivative, thiamine tetrahydrofurfuryl disulfide (TTFD), is better absorbed than readily-available water-soluble thiamine salts because it does not require the rate-limiting transport system required for thiamine absorption. However, the detailed pharmacokinetics of thiamine and TTFD under normal and pathological conditions have not yet been clarified. C-11-labeled thiamine and TTFD were recently synthesized by our group. In this study, to clarify the differences in pharmacokinetics and metabolism of these probes, a quantitative PET imaging study and radiometabolite analysis of C-11-labeled thiamine and TTFD were performed in the rat heart.ProceduresPositron emission tomography (PET) imaging with [C-11]thiamine and [C-11]TTFD was performed in normal rats to determine the pharmacokinetics of these probes, and the radiometabolites of both probes from the blood and heart tissue were analyzed by thin-layer chromatography.ResultsAccumulation of [C-11]TTFD was significantly higher than that of [C-11]thiamine in the rat heart. Moreover, as a result of the radiometabolite analysis of heart tissue at 15min after the injection of [C-11]TTFD, thiamine pyrophosphate, which serves as a cofactor for the enzymes involved in glucose metabolism, was found as the major radiometabolite and at a significantly higher level than in the [C-11]thiamine-injected group.ConclusionsPET imaging techniques for visualizing the kinetics and metabolism of thiamine using [C-11]thiamine and [C-11]TTFD were developed in this study. Consequently, noninvasive PET imaging for the pathophysiology of thiamine-related cardiac function may provide novel information about heart failure and related disorders.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.4
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available