Journal
MOLECULAR CELL
Volume 70, Issue 2, Pages 254-+Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2018.03.015
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Funding
- Intramural Research Program of the NIH, NICHD
- Science Foundation Ireland [13/IA/1853, 12/IP/1492]
- Health Research Board Cancer scholarship
- Science Foundation Ireland (SFI) [12/IP/1492, 13/IA/1853] Funding Source: Science Foundation Ireland (SFI)
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Translation initiation is typically restricted to AUG codons, and scanning eukaryotic ribosomes inefficiently recognize near-cognate codons. We show that queuing of scanning ribosomes behind a paused elongating ribosome promotes initiation at upstream weak start sites. Ribosomal profiling reveals polyamine-dependent pausing of elongating ribosomes on a conserved Pro-Pro-Trp (PPW) motif in an inhibitory non-AUG-initiated upstream conserved coding region (uCC) of the antizyme inhibitor 1 (AZIN1) mRNA, encoding a regulator of cellular polyamine synthesis. Mutation of the PPW motif impairs initiation at the uCC's upstream near-cognate AUU start site and derepresses AZIN1 synthesis, whereas substitution of alternate elongation pause sequences restores uCC translation. Impairing ribosome loading reduces uCC translation and paradoxically derepresses AZIN1 synthesis. Finally, we identify the translation factor eIF5A as a sensor and effector for polyamine control of uCC translation. We propose that stalling of elongating ribosomes triggers queuing of scanning ribosomes and promotes initiation by positioning a ribosome near the start codon.
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