Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 29, Issue 8, Pages 886-896Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E17-12-0729
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Funding
- National Institutes of Health (NIH) [GM051173, GM1100413, GM083122, HL128370]
- Children's Discovery Institute [PD-II-2014-379]
- Greater Southeast Affiliate Pre-doctoral Fellowship from American Heart Association [10PRE353007]
- Biochemistry, Cell, and Molecular Biology (BCMB) Training Grant at Emory University (NIH) [T32 GM008367]
- Emory University Catalyst Grant
- Emory Integrated Genomics Core (EIGC)
- Emory University School of Medicine
- Emory Integrated Core Facilities
- Japan Society for the Promotion of Science (JSPS)
- National Center for Advancing Translational Sciences of the National Institutes of Health [UL1TR000454]
- NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES [UL1TR002378, UL1TR000454] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL128370] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM051173, R37GM051173, T32GM008367, R01GM083122] Funding Source: NIH RePORTER
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Axonemal dyneins, including inner dynein arm I1, assemble in the cytoplasm prior to transport into cilia by intraflagellar transport (IFT). How I1 dynein interacts with IFT is not understood. We take advantage of the Chlamydomonas reinhardtii ida3 mutant, which assembles the inner arm I1 dynein complex in the cytoplasm but fails to transport I1 into the cilium, resulting in I1 dynein-deficient axonemes with abnormal motility. The IDA3 gene encodes an similar to 115-kDa coiled-coil protein that primarily enters the cilium during ciliary growth but is not an axonemal protein. During growth, IDA3, along with I1 dynein, is transported by anterograde IFT to the tip of the cilium. At the tip, IDA3 uncouples from IFT and diffuses within the cilium. IFT transport of IDA3 decreases as cilia lengthen and subsides once full length is achieved. IDA3 is the first example of an essential and selective IFT adapter that is regulated by ciliary length.
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