Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 473, Issue -, Pages 217-224Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2018.01.022
Keywords
Testis; Anti-inflammatory drugs; GDNF; Male fertility; Male infertility
Categories
Funding
- Deutsche Forschungsgemeinschaft (DFG) [MA1080/25-1]
- CONICET
- Deutscher Akademischer Austauschdienst (DAAD) [57214227]
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In man, blockage of prostaglandin (PG)-production e.g. by non-steroidal anti-inflammatory drug (NSAIDs) may have negative testicular side effects, implying beneficial actions of PGs in the testis. We examined human testicular samples and isolated human testicular peritubular cells (HTPCs) to explore sites of PG-synthesis and targets. HTPCs express cyclooxygenase 1 (COX1) and secrete PGE(2). Receptors (EP1, 2, 4) were specifically identified in peritubular cells. In HTPCs PGE(2) significantly increased mRNA levels of the contractility protein calponin, but did not induce contractions. PGE(2), as well as EP1 and EP4 receptor agonists, significantly increased glia cell line derived neurotrophic factor (GDNF) mRNA and/or protein levels. Importantly, the NSAID ibuprofen reduced PGE(2) and this action also lowered SMA and calponin mRNA levels and levels of secreted GDNF protein. The results reveal an unknown PGE(2) system in the human testis, in involving peritubular cells, which may be prone to interference by NSAIDs. (C) 2018 Elsevier B.V. All rights reserved.
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