Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 38, Issue 9, Pages -Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.00672-17
Keywords
beta 1-integrin; homologous recombination; HR; Rad51; breast cancer; radioresistance
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Funding
- Houston Methodist Research Institute
- MD Anderson Cancer Center, Houston, TX
- National Institutes of Health [CA129537, GM109768]
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The molecular mechanisms underlying resistance to radiotherapy in breast cancer cells remain elusive. Previously, we reported that elevated beta 1-integrin is associated with enhanced breast cancer cell survival postirradiation, but how beta 1-integrin conferred radioresistance was unclear. Ionizing radiation (IR) induced cell killing correlates with the efficiency of DNA double-strand break (DSB) repair, and we found that nonmalignant breast epithelial (S1) cells with low beta 1-integrin expression have a higher frequency of S-phase-specific IR-induced chromosomal aberrations than the derivative malignant breast (T4-2) cells with high beta 1-integrin expression. In addition, there was an increased frequency of IR-induced homologous recombination (HR) repairosome focus formation in T4-2 cells compared with that of S1 cells. Cellular levels of Rad51 in T4-2 cells, a critical factor in HR-mediated DSB repair, were significantly higher. Blocking or depleting beta 1-integrin activity in T4-2 cells reduced Rad51 levels, while ectopic expression of beta 1-integrin in S1 cells correspondingly increased Rad51 levels, suggesting that Rad51 is regulated by beta 1-integrin. The low level of Rad51 protein in S1 cells was found to be due to rapid degradation by the ubiquitin proteasome pathway (UPP). Furthermore, the E3 ubiquitin ligase RING1 was highly upregulated in S1 cells compared to T4-2 cells. Ectopic beta 1-integrin expression in S1 cells reduced RING1 levels and increased Rad51 accumulation. In contrast, beta 1-integrin depletion in T4-2 cells significantly increased RING1 protein levels and potentiated Rad51 ubiquitination. These data suggest for the first time that elevated levels of the extracellular matrix receptor beta 1-integrin can increase tumor cell radioresistance by decreasing Rad51 degradation through a RING1-mediated proteasomal pathway.
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