Journal
MOLECULAR & CELLULAR PROTEOMICS
Volume 17, Issue 7, Pages 1337-1353Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/mcp.RA118.000623
Keywords
Nuclear Translocation; SILAC; Mass Spectrometry; Cell biology; Affinity proteomics; eIF1A; importin 13; nucleus; Ubc9
Categories
Funding
- Deutsche Forschungsgemeinschaft (DFG) [KE 660/14-1]
- Sonderforschungsbereich [SFB 860]
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Importin 13 is a member of the importin beta family of transport receptors. Unlike most family members, importin 13 mediates both, nuclear protein import and export. To search for novel importin 13 cargoes, we used stable isotope labeling of amino acids in cell culture (SILAC) and mass spectrometry. Using stringent criteria, we identified 255 importin 13 substrates, including the known cargoes Ubc9, Mago and eIF1A, and validate many of them as transport cargoes by extensive biochemical and cell biological characterization. Several novel cargoes can also be transported by the export receptor CRM1, demonstrating a clear redundancy in receptor choice. Using importin 13 mutants, we show that many of the novel substrates contact regions on the transport receptor that are not used by Ubc9, Mago or eIF1A. Together, this study significantly expands the repertoire of importin 13 cargoes and sets the basis for a more detailed characterization of this extremely versatile transport receptor.
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