Journal
MICRON
Volume 106, Issue -, Pages 21-26Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micron.2017.12.007
Keywords
Anatomy; Electron microscopy; Exoskeleton; Methods; Morphology; Ultrastructure
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Funding
- CAL, University Roma Tre, Rome, IT
- Department of Science of Roma Tre University
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Transmission Electron Microscopy (TEM) has always been the conventional method to study arthropod ultra structure, while the use of Scanning Electron Microscopy (SEM) was mainly devoted to the examination of the external cuticular structures by secondary electrons. The new generation field emission SEMs are capable to generate images at sub-cellular level, comparable to TEM images employing backscattered electrons. The potential of this kind of acquisition becomes very powerful in the dual beam FIB/SEM where the SEM column is combined with a Focused Ion Beam (FIB) column. FIB uses ions as a nano-scalpel to slice samples fixed and embedded in resin, replacing traditional ultramicrotomy. We here present two novel methods, which optimize the use of FIB/SEM for studying arthropod anatomy.
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