Journal
MICROCHIMICA ACTA
Volume 185, Issue 8, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-018-2920-5
Keywords
Copper nanoclusters; Fluorescence; Turn-off-on; Electron transfer; Stern-Volmer equation; Penicillamine
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Funding
- National Natural Science Foundation of China [21707030]
- Wuhan Youth Science and technology plan [2016070204010133]
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Stable copper nanoclusters (CuNCs) were prepared by utilizing D-penicillamine as both the stabilizer and reductant. The emission of the CuNCs (with excitation/emission peaks at 390/645 nm) is largely stabilized by coating with poly(sodium-p-styrenesulfonate) (PSS). Cytochrome c (Cyt c) quenches the fluorescence of the PSS-coated CuNCs, and this effect was exploited to design a quenchometric fluorometric assay for Cyt c. If trypsin is added to the loaded CuNCs, it will hydrolyze Cyt c to form peptide fragments, and fluorescence is gradually restored. A highly sensitive and fluorometric turn-off-on assay was constructed for sequential detection of Cyt c and trypsin. The linear ranges for Cyt c and trypsin are from 8.0 nM to 680 nM, and from 0.1 to 6.0 mu g mL(-1), and the lower detection limits are 0.83 nM and 20 ng mL(-1) for Cyt c and trypsin, respectively.
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