Journal
MICROCHIMICA ACTA
Volume 185, Issue 8, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-018-2912-5
Keywords
Michaelis constant; Substrate saturation; Hydrogen-peroxide: Hydrogen-peroxide oxidoreductase; H2O2; Catalase; Colloidal semiconductor nanocrystals; Chemiluminescence assay; CdTe quantum dots
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Funding
- research council of Tarbiat Modares University
- Ministry of Sciences, Researches, and Technology
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A method is described for the chemiluminescence based determination of the activity of catalase (CAT) using H2O2-sensitive CdTe quantum dots (QDs). It is based on the finding that the chemiluminescence (CL) of the CdTe/H2O2 system is reduced due to the consumption of H2O2 by the catalytic action of CAT. The Michaelis constant is calculated to be 519 +/- 27 mM, showing the potential of the method to accurately measure the K-m compared to the standard method. The method does not require QDs to be conjugated to biological/organic molecules and therefore is considered to be a rapid and convenient method for determination of CAT in real samples. At an incubation time of 2 s, the LOD was calculated to be 4.5 unit/mL, with a linear range from 6 to 400 unit/mL. The assay is sensitive, simple, and suitable for practical applications.
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