Journal
MICROCHIMICA ACTA
Volume 185, Issue 2, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-018-2683-z
Keywords
Fluorescence; Aptamer; Carbon dots; Adenosine triphosphate; Yogurt; Fluorescence resonance energy transfer; Dairy product; Food safety; Food analysis; Quenching
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Funding
- National Natural Science Foundation of China [61775099, 21705080]
- Natural Science Foundation of Jiangsu Province [BK20171487, BK20171043]
- Natural Science Foundation of the Higher Education Institutions of Jiangsu Province [17KJB150029]
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The authors describe a fluorometric aptamer based assay for adenosine triphosphate (ATP). It is based on the use of carbon dots (CDs) and graphene oxide (GO). The resultant CD-aptamer is adsorbed on the surface of GO via p-stacking and hydrophobic interaction, and the fluorescence of CD-aptamer is quenched via fluorescence resonance energy transfer (FRET) between CDs and GO. If ATP is present, it will bind to the aptamer and the CD-aptamer will be desorbed from GO. This will suppress FRET and the fluorescence of the CDs is restored. Under the optimal conditions and at typical excitation/emission wavelengths of 358/455 nm, the assay has a 80 pM detection limit and a linear range that extends from 0.10 to 5.0 nM concentrations of ATP. The method was successfully applied to the determination of ATP in yogurt samples. This method can also be conceivably applied to the detection of other analytes for which appropriate aptamers are available.
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