Journal
MICROCHIMICA ACTA
Volume 185, Issue 8, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-018-2896-1
Keywords
Food safety; Visual detection; de-aggregation; Immunomagnetic separation; Nanozyme; o-phenylenediamine
Categories
Funding
- Chinese National Natural Science Foundation [81602894, 81602895, 81473018]
- China Postdoctoral Science Foundation [2017 T100214, 2016 M591492]
- Development Foundation of Science and Technology in Jilin Province of China [20170204003SF]
- Graduate Innovation Fund of Jilin University [2017084]
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The authors describe a rapid colorimetric assay for Listeria monocytogenes (L. monocytogenes) based on the o-phenylenediamine-mediated deaggregation of gold nanoparticles. Silver nanoclusters are used as an artificial enzyme that can oxidize o-phenylenediamine to form o-benzoquinone diamine. Aptamer and IgY antibodies were chosen to conjugate with magnetic beads and silver nanoclusters, respectively, which can recognize and bind L. monocytogenes at different specific binding sites. This results in the disassembly of colloidal gold nanoparticles which is accompanied by a color change from blue to red, with peaks at 730 and 525 nm, respectively. The method allows L. monocytogenes to be colorimetrically determined in the 10 to 10(6) cfu center dot mL(-1) concentration range without pre-enrichment, and the limit of detection is as low as 10 cfu center dot mL(-1). Recoveries ranging from 97.4 to 101.3% are found when analyzing spiked food samples. The assay is rapid, sensitive and specific.
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