Journal
MICROCHIMICA ACTA
Volume 185, Issue 2, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-017-2662-9
Keywords
Raman spectroscopy; Citrate-capped AgNPs; Aggregation agent; Attomolar detection
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Funding
- Ministry of Science And Technology, Taiwan
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Making good use of interactions between analyte molecules and the metal nanoparticles is key to impact the detection limit in a surface-enhanced Raman scattering (SERS) based detections. SERS was applied to the analysis of catechin and it was found that the relative abundance of catechin in the sample to citrate-capped AgNPs and the aggregation agent NaCl plays a critical role in the quality of detection. At a component volume ratio of 6: 2: 1 (catechin: AgNPs: NaCl), catechin can be detected at mu M levels. When the ratio is 12: 2: 1, Raman signals are discernible even at the attomolar concentration level (10(-18) M). Under these conditions, the SERS mechanisms and the force of laser tweezers function best. The extent of signal enhancement enabled an ultrasensitive and reproducible Raman spectroscopic determination of catechin.
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