Journal
MICROCHIMICA ACTA
Volume 185, Issue 7, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-018-2901-8
Keywords
AuNPs; ATP; Curcubit[7]uril; Surface energy transfer; Fluorescence; Rhodamine B; Nanosensing; Supramolecular functionalization
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Funding
- Lebanese National Council of Scientific Research (NCSR), Lebanon
- American University of Beirut, Lebanon through URB, K. Shair CRSL endowed research fund
- Kamal A. Shair Central Research Laboratory (KAS CRSL)
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The authors describe a method for functionalization of gold nanoparticles (AuNPs) with the supramolecular host molecule, curcubit[7]uril (CB[7]) which can bind rhodamine B (RhB). The fluorescence of RhB is quenched by the AuNPs via surface energy transfer. On addition of ATP, a dimeric RhB-ATP complex is formed and RhB is pushed out of CB[7]. Hence, fluorescence increases by a factor of 8. This fluorescence recovery effect has been utilized to develop a new detection scheme for ATP. The assay, measured at fluorescence excitation and emission wavelengths of 500 nm and 574 nm respectively, works in the 0.5-10 mu M concentration range and has a 100 nM detection limit. The method is not interfered by UTP, GTP, CTP, TTP, ascorbic acid and glutathione.
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