Journal
MICROCHIMICA ACTA
Volume 185, Issue 3, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-018-2689-6
Keywords
Naphthalimide; Magnetic enrichment; Quencher; Fluorescent labeling; Amplify; Hybridization; Nanoparticles; Mercury ions
Categories
Funding
- National Natural Science Foundation of China [21507034, 51521006]
- Natural Science Foundation of Guangdong Province, China [2016A030310022]
- Pearl River S&T Nova Program of Guangzhou [201710010134]
Ask authors/readers for more resources
The authors describe a fluorometric assay for the determination of Hg(II). A naphthalimide derivative is used as a label for a thymine (T) rich ssDNA, and graphene oxide magnetized with Fe3O4 nanoparticles acts as a quencher and preconcentrators. In the absence of Hg(II), the labeled ssDNA does not separate from the magnetized graphene oxide. As a result, fluorescence is fully quenched. In the presence of Hg(II), a T-Hg(II)-T link is formed dues to the highly affinity between T and Hg(II). Hence, fluorescence is restored. The assay has a linear response in the 1.0 to 10.0 nM Hg(II) concentration range, and a 0.65 nM detection limit. The method is selective and sensitive. It was applied to the analysis of spiked environmental water samples, and data agreed well with those obtained by atomic fluorescence spectrometry.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available