Use of chicken feet yellow membrane as a biosorbent in miniaturized solid phase extraction for determination of polycyclic aromatic hydrocarbons in several real samples

In this study, chicken feet yellow membrane was treated and evaluated as a novel bio-sorbent for miniaturized solid-phase extraction of organic pollutants and determination by high performance liquid chromatography for the first time. The surface of bio-sorbent particles was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction and Brunauer, Emmett and Teller techniques. As-prepared biosorbent showed excellent potential for preconcentration of polycyclic aromatic hydrocarbons (PAHs) as a model of organic pollutants. The effective experimental parameters on the extraction efficiency were optimized by onefactor-at-a-time method. Under the optimized experimental conditions, linear dynamic ranges were achieved in the range of 0.1-400.0 mu g L-1 for Naphtalene, 0.005-40.0 mu g L-1 for Phenanthrene, 0.001-20.0 mu g L-1 for Antracene, 0.008-132.0 mu g L-1 for Fluoranthene and 0.012-100.0 mu g L-1 for Pyrene, with correlation coefficient ranging from 0.996 to 0.999. The limits of detection for PAHs varied between 0.37 and 38.5 ng L-1 and RSD values were obtained in the range of 2.7-5.4% (n = 8). The applicability of the method was demonstrated by analyzing the target pollutants in agricultural products, edible vegetable oils, and water samples. Due to low cost, availability, environmentally friendly, reusability and good pre-concentration factors, the developed biosorbent could be effectively used for the determination of PAHs in environmental samples.