Preparation and evaluation of a polar embedded diphenylethene bonded stationary phase for High Performance Liquid Chromatography

A new polar embedded diphenylethene bonded stationary phase (click DIP) was prepared via click chemistry and evaluated. This new material was characterized by FT-IR spectroscopy, elemental analysis and thermal gravimetric analysis. Alkylbenzenes and polycyclic aromatic hydrocarbons (PAHs) were separated in reverse-phase mode. Van't Hoff plot and linear solvation energy relationships model were used to investigate the separation mechanism and properties of the new column, the result shows that hydrophobic and pi-pi interaction are important for it to retain solutes. The packed column was employed to separate high polar solutes like carboxylic acids and nucleobase. Moreover, three isomers of terphenyl and medicine solutes including five sulfonamides and four local anaesthetics were successfully separated, much better performed than conventional C18 column. These results proved that this new stationary phase may possess great potential in environmental and pharmaceutical analysis.