Journal
MICROBIOLOGY
Volume 87, Issue 1, Pages 47-50Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0026261718010022
Keywords
gibberellic acid; plant hormone; aerobic methylotroph; Methylobacillus arboreus; plant symbiont
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Funding
- Russian Foundation for Basic Research [16-04-00381_a]
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An obligate methylotroph Methylobacillus arboreus Iva(T) (VKM B-2590(T), CCUG 59684(T), DSM 23628(T)) is the first known aerobic methylotrophic bacterium capable of synthesis of the bioactive gibberellic acid GA(3). Primary separation and identification of gibberellic acid from the culture liquid of methanol-grown culture were carried out using thin-layer chromatography and high-performance liquid chromatography. The concentration and structure of the gibberellic acid GA(3) were determined by liquid chromatography-mass spectrometry (LC/MS). Biological activity of the isolated compound was confirmed by tests on sprouts of lettuce (Lactuca sativa L.).
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